crisper bacteria breakthroughs
- 6 years ago
DNA is an excellent medium for archiving data. Recent efforts have illustrated the potential for information storage in DNA using synthesized oligonucleotides assembled in vitro1,2,3,4,5,6. A relatively unexplored avenue of information storage in DNA is the ability to write information into the genome of a living cell by the addition of nucleotides over time. Using the Cas1–Cas2 integrase, the CRISPR–Cas microbial immune system stores the nucleotide content of invading viruses to confer adaptive immunity.
When harnessed, this system has the potential to write arbitrary information into the genome8. Here we use the CRISPR–Cas system to encode the pixel values of black and white images and a short movie into the genomes of a population of living bacteria. In doing so, we push the technical limits of this information storage system and optimize strategies to minimize those limitations.
RESEARCH PAPER'S :
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1144-4
https://www.ncbi.nlm.nih.gov/pubmed/27194423
https://www.nature.com/news/modified-viruses-deliver-death-to-antibiotic-resistant-bacteria-1.22173
http://www.cell.com/cell-stem-cell/fulltext/S1934-5909(17)30274-6
https://www.nature.com/nature/journal/v547/n7663/full/nature23017.html
http://advances.sciencemag.org/content/3/4/e1602814
https://link.springer.com/article/10.1007%2Fs00438-017-1299-z
http://stm.sciencemag.org/content/8/360/360ra134
When harnessed, this system has the potential to write arbitrary information into the genome8. Here we use the CRISPR–Cas system to encode the pixel values of black and white images and a short movie into the genomes of a population of living bacteria. In doing so, we push the technical limits of this information storage system and optimize strategies to minimize those limitations.
RESEARCH PAPER'S :
https://genomebiology.biomedcentral.com/articles/10.1186/s13059-016-1144-4
https://www.ncbi.nlm.nih.gov/pubmed/27194423
https://www.nature.com/news/modified-viruses-deliver-death-to-antibiotic-resistant-bacteria-1.22173
http://www.cell.com/cell-stem-cell/fulltext/S1934-5909(17)30274-6
https://www.nature.com/nature/journal/v547/n7663/full/nature23017.html
http://advances.sciencemag.org/content/3/4/e1602814
https://link.springer.com/article/10.1007%2Fs00438-017-1299-z
http://stm.sciencemag.org/content/8/360/360ra134